Inverted
ILM Flap Technique
Zofia Michalewska, Jerzy Nawrocki
Pak J Ophthalmol 2019, Vol. 35, No.
3
As recently as 30 years ago, macular holes were
thought to be untreatable. Kelly and Wendel showed that this was not the case
in 1991 when they published their pilot study on vitreous surgery for
idiopathic macular holes1. In 1997, Eckardt et al. introduced
internal limiting membrane (ILM) peeling2, following which, pars
plana vitrectomy, internal limiting membrane peeling, fluid/gas exchange and
face-down positioning were soon adopted as the standard treatment method for
successful hole closure and BCVA improvement. Surgeons improved their own
results by using smaller gauge instruments and, especially, by more qualified
patient selection: earlier treatment, non-myopic eyes, better initial visual acuity,
and smaller macular holes.
Despite these huge advances, 100% success rates of the
classical approach, briefly described above, that were often quoted in the past
were perhaps overly optimistic. Modern OCT-based studies reveal that 19-39% of
cases actually result in “flat open” anatomical outcomes (also referred to in
the literature as type two closure), where the margins of the macular hole remain flat with bare retinal
pigment epithelium. Although the hole will normally not increase in size, visual
acuity does not improve in these cases thus the surgical intervention cannot be
said to have been fully successful3. OCT images reveal U-Type,
V-Type and irregular closure are the three successful macular hole anatomical
closure types that also provide better functional improvement.
After careful investigation of OCT images from “flat
open” results, Jerzy Nawrocki came to the conclusion that ILM tissue has an
actual role to play in ensuring that the macular hole is successfully closed.
As a base membrane, its properties enable cell proliferation and reactive
gliosis. Inverting the ILM and laying it over the macular hole on the surface
of the retina may create a scaffold, over which Müller cells can spread and
photoreceptor cells may follow the gliosis, either re-approximating or
re-growing over time. The first surgeries with the “Inverted ILM Flap Technique”
were carried out between 2006 and 2009 and the highly encouraging results were
presented at the American Society of Retinal Specialists (ASRS), European
Vitreo-retinal Society (EVRS) and German Ophthalmological Society (DOG)
meetings. Our first papers on the
inverted flap technique were published in 2009 and 20104,5.
The technique itself was modified in 2015 when we
introduced the “Temporal Inverted ILM Flap Technique” in order to reduce overall
trauma to the nerve fibres thereby reducing the amount of dissociated optic
nerve fibre layer (DONFL), described by Tadayoni et al6 and to help
prevent the flap moving away from the macular hole during fluid/air exchange7.
It is a straightforward procedure although the manoeuvres involving
manipulating the flap can take some time to perfect. Core vitrectomy is
performed and posterior hyaloid detached and removed. Trypan blue is applied for
40 seconds to 1 minute to aid visualisation of the ILM, which is peeled on the
temporal side of the macular hole but it is not completely removed. A
sufficiently large piece of ILM is left attached at the margins of the hole and
turned over and inverted so that the surface previously facing the vitreous
body is now facing the retinal pigment epithelium. It is gently massaged over
the macular hole (keeping it on top of the retina) and is held in place with
fluid/air injection (with the flow moving from the attached end of the ILM flap
towards the free end). Patients are asked to maintain a position where the air
bubble stays in the centre of their visual field for 3 days.
The flap appears to perform its intended role as a
scaffold, as confirmed immunohistochemically by Shiode et al8. Gliosis,
migration and proliferation of Müller cells, microstructural regeneration,
decreased ellipsoid zone defects and the macular hole closing and filling with
tissue have all been reported along with BCVA improvements of 1-4 lines - as
early as one day after surgery9.
Since its introduction, the flap technique has been
investigated by many surgeons across the world and various authors have
introduced their own adaptations and modifications. These have included filling
the hole with attached ILM (which has led to the original techniques proposed
by our group being called a “cover” technique and the others “fill” or
“stuffing” techniques). Others have used free flaps of unattached ILM, created
multiple flaps, transplanted ILM to cover or fill the hole, used a lens
capsular flap, or an amniotic membrane flap. Different dyes have been used and
alternative methods to hold the flap in place such as viscoelastic and
gluconated blood have been reported in the literature10-14.
As with any invasive surgical technique and any
intervention involving dyes, there are potential risks involved when using the
various flap techniques. Comparative studies of the Inverted ILM Flap Technique
(“cover” techniques), “fill” techniques and classical ILM peeling seem to show that
the Inverted flap technique is a highly effective surgical method to close large
macular holes and restore retinal tissue and improve BCVA5,15.
Initially developed to deal with large macular holes
the inverted flap technique tends to be used more often to treat large, old and
myopic macular holes and for reoperations after conventional surgery has
failed. However, it has also been adopted to treat routine small cases and has
successfully resolved macular hole with retinitis pigmentosa, in an eye with Coat’s
Disease, (both unpublished) and a case of secondary rupture of a retinal
arterial macroaneurysm16, as well as to successfully treat
persistent macular hole after retinal detachment surgery, macular holes with
AMD, traumatic macular holes, diabetic macular holes, macular holes secondary
to uveitis and optic pit maculopathy.
SD and SS OCT show foveal microstructure recovery
following surgery with the inverted flap technique. Hayashi
and Kuriyama found reappearance of the ellipsoid zone and ELM during 6-months-follow
up (more frequently in large and myopic cases compared to macular hole retinal
detachment)17. Despite finding expansion of
submacular pigment epithelium atrophy during follow-up, Imai and Azumi reported
visual acuity improvement 0.08 to 0.3 (Landolt C)18. Chen et al. found improvement in multifocal electroretinography after
the inverted flap technique for large macular hole and although it was not
significantly correlated with BCVA it may be a useful supplement when
evaluating functional recovery19.
A new macular hole closure type: “flap closure” has
recently been described by Bonińska, Nawrocki and Michalewska. In these
cases, large macular holes with lower preoperative visual acuity which probably
would not have been closable without the use of the inverted flap technique, SD
or SS-OCT revealed a thin flap of tissue that had closed the hole one week
after surgery. Foveal architecture continuously restored up to one year
follow-up and mean visual acuity improved. However, final visual acuity was
lower when compared to eyes with initial U- type, V-type or irregular closure20.
Whether or not the inverted flap
technique should be considered as the first approach for the treatment of all
macular holes is still open to discussion. The published outcomes of surgery as
well as the comparative studies published to date have meanwhile led to the
inverted flap technique and its adaptations being adopted by a significant
number of ophthalmic surgeons throughout the world.
REFERENCES
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Gaudric A. Dissociated optic nerve fiber layer appearance of the fundus
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Dulczewska-Cichecka K, Adelman RA, Nawrocki J. Temporal inverted internal
limiting membrane flap technique versus classic inverted internal limiting
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8. Shiode
Y, Morizane
Y, Matoba
R, Hirano
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K, Araki
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p.97-113.
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17. Hayashi H, Kuriyama S. Foveal microstructure in macular holes
surgically closed by inverted internal limiting membrane flap technique. Retina,
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18. Imai H, Azumi A. The expansion of RPE
atrophy after inverted ILM flap technique for a chronic large macular hole.
Case Rep Ophthalmol. 2014; 5 (1): 83-6.
19. Chen Z, Zhao C, Ye JJ, Wang XQ, Sui RF.
Inverted Internal Limiting Membrane Flap Technique for Repair of Large Macular
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Author’s Affiliation
Zofia
Michalewska, MD, PhD
Ophthalmic Clinic “Jasne Blonia”.
Ul. Rojna 90. Lodz, 91-162, Poland.
Tel: +48 (0)42 636 8282 ; Fax: +48 (0)42 611 0505
E-mail: zosia_n@yahoo.com
Jerzy Nawrocki
MD, PhD.
Ophthalmic Clinic
“Jasne Blonia”
Rojna 90, Lodz,
Poland.